gamma Adaptin Recombinant Rabbit Monoclonal Antibody [PSH02-65]
cat.: HA721843
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH02-65 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 91 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human gamma Adaptin aa 623-822 / 822. |
| Positive control: | HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, HepG2 cell lysate, Raji cell lysate, MCF7 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse kidney tissue lysate, mouse liver tissue lysate, rat kidney tissue lysate, rat liver tissue lysate, mouse kidney tissue, rat brain tissue. |
| Subcellular location: | Golgi apparatus, Cytoplasmic vesicle, clathrin-coated vesicle membrane, Cytoplasm, perinuclear region, |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: O43747 Human | P22892 Mouse | A0A8I5Y697 Rat |
| Alternative names: | Adapter-related protein complex 1 subunit gamma-1 Adaptor protein complex AP 1 gamma 1 subunit Adaptor protein complex AP 1 subunit gamma 1 Adaptor protein complex AP-1 subunit gamma-1 Adaptor related protein complex 1 gamma 1 subunit ADTG AP 1 complex subunit gamma 1 AP-1 complex subunit gamma-1 AP1G1 AP1G1_HUMAN CLAPG1 Clathrin assembly protein complex 1 gamma 1 large chain Clathrin assembly protein complex 1 gamma large chain Clathrin assembly protein complex 1 gamma-1 large chain Clathrin associated/assembly/adaptor protein large gamma 1 Gamma1 adaptin Gamma1-adaptin Golgi adaptor HA1/AP1 adaptin gamma subunit Golgi adaptor HA1/AP1 adaptin subunit gamma 1 Golgi adaptor HA1/AP1 adaptin subunit gamma-1 MGC18255 |
Images
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Fig1:
Western blot analysis of gamma Adaptin on different lysates with Rabbit anti-gamma Adaptin antibody (HA721843) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Lane 5: Raji cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: A549 cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: RAW264.7 cell lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (20 µg/Lane) Lane 11: Mouse kidney tissue lysate (40 µg/Lane) Lane 12: Mouse liver tissue lysate (40 µg/Lane) Lane 13: Rat kidney tissue lysate (40 µg/Lane) Lane 14: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 91 kDa Observed band size: 91 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721843) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-gamma Adaptin antibody (HA721843) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721843) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-gamma Adaptin antibody (HA721843) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721843) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Western blot analysis of gamma Adaptin on different lysates with Rabbit anti-gamma Adaptin antibody (HA721843) at 1/1,000 dilution. Lane 1: HEK-293-si NT cell lysate Lane 2: HEK-293-si gamma Adaptin cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 91 kDa Observed band size: 91 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721843) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.