c17orf74 Recombinant Rabbit Monoclonal Antibody [PSH02-66]
cat.: HA721844
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: PSH02-66
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 57 kDa
Isotype: IgG
Immunogen: Recombinant protein within human c17orf74 aa 202-501 / 501.
Positive control: Human testis tissue, mouse testis tissue, rat testis tissue.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:200
Uniprot #: SwissProt: Q0P670 Human | Q8C5U4 Mouse | Q68FV4 Rat
Alternative names: Uncharacterized protein SPEM2 SPEM2 C17orf74 SPEM family member 2
Images
HA721844_1.jpg Fig1: Western blot analysis of c17orf74 on different lysates with Rabbit anti-c17orf74 antibody (HA721844) at 1/1,000 dilution.

Lane 1: HEK-293-NT cell lysate
Lane 2: HEK-293-OE-c17orf74 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 57 kDa
Observed band size: 57 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721844) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721844_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-c17orf74 antibody (HA721844) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721844) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721844_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-c17orf74 antibody (HA721844) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721844) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721844_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-c17orf74 antibody (HA721844) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721844) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.