MAN2A1 Recombinant Rabbit Monoclonal Antibody [PSH02-67]
cat.: HA721845
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH02-67 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 131 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MAN2A1 aa 51-500 / 1,144. |
| Positive control: | HepG2 cell lysate, Huh7 cell lysate, JAR cell lysate, U-87 MG cell lysate, HUVEC cell lysate, A375 cell lysate, MDA-MB-468 cell lysate, NCI-H226 cell lysate, human liver tissue lysate, human lung tissue lysate, human kidney tissue lysate, human colon cancer tissue, human stomach tissue, U-87 MG. |
| Subcellular location: | Golgi apparatus membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q16706 Huamn |
| Alternative names: | 3-1 6-alpha-mannosidase Alpha mannosidase 2 alpha Mannosidase II Alpha-mannosidase 2 AMAN II Golgi alpha mannosidase II Golgi alpha-mannosidase II Golgi integral membrane protein 7 GOLIM7 MA2A1_HUMAN MAN II Man2a1 MANA 2 MANA2 MANII Mann II Mannosidase alpha class 2A member 1 Mannosidase Two Mannosidase, alpha type II Mannosidase, alpha, II Mannosyl oligosaccharide 1 3 1 6 alpha mannosidase Mannosyl oligosaccharide 1,3 1,6 alpha mannosidase Mannosyl-oligosaccharide 1 |
Images
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Fig1:
Western blot analysis of MAN2A1 on different lysates with Rabbit anti-MAN2A1 antibody (HA721845) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Huh7 cell lysate Lane 3: JAR cell lysate Lane 4:U-87 MG cell lysate Lane 5: HUVEC cell lysate Lane 6: A375 cell lysate Lane 7: MDA-MB-468 cell lysate Lane 8: NCI-H226 cell lysate Lane 9: Human liver tissue lysate Lane 10: human lung tissue lysate Lane 11: human kidney tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 131 kDa Observed band size: 131-151 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721845) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-MAN2A1 antibody (HA721845) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721845) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-MAN2A1 antibody (HA721845) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721845) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of U-87 MG cells labeling MAN2A1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA721845, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.