MPP1 Recombinant Rabbit Monoclonal Antibody [PSH02-69]
cat.: HA721847
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell
Clonality: Monoclonal
Clone number: PSH02-69
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 53 kDa
Isotype: IgG
Immunogen: Recombinant protein within human MPP1 aa 1-466 / 466.
Positive control: K-562 cell lysate, Jurkat cell lysate, HEK-293 cell lysate, HeLa, human heart tissue, mouse heart tissue, rat heart tissue.
Subcellular location: Cell membrane, Cell projection, stereocilium.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
1:1,000
1:1,000
1:100
Uniprot #: SwissProt: Q00013 Human | P70290 Mouse
Entrez Gene: 652956 Rat
Alternative names: 55 kDa erythrocyte membrane protein AAG 12 AAG12 Aging associated gene 12 DXS552 DXS552E EM55_HUMAN EMP 55 EMP55 Erythrocyte membrane protein p55 Membrane protein Membrane protein palmitoylated 1 55kDa Membrane protein palmitoylated 1 Membrane protein, palmitoylated 1, 55kDa Migration related gene 1 MPP 1 MPP1 MRG 1 MRG1 OTTHUMP00000026054 OTTHUMP00000196176 p55 palmitoylated 1 Palmitoylated erythrocyte membrane protein Palmitoylated membrane protein 1 PEMP
Images
HA721847_1.jpg Fig1: Western blot analysis of MPP1 on different lysates with Rabbit anti-MPP1 antibody (HA721847) at 1/1,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HEK-293 cell lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 52 kDa
Observed band size: 53 kDa

Exposure time: 5 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721847) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721847_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling MPP1 with Rabbit anti-MPP1 antibody (HA721847) at 1/100 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MPP1 antibody (HA721847) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721847_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-MPP1 antibody (HA721847) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721847) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721847_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-MPP1 antibody (HA721847) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721847) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721847_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-MPP1 antibody (HA721847) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721847) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.