RHOG Recombinant Rabbit Monoclonal Antibody [PSH02-70]
cat.: HA721848
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH02-70 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human RHOG aa 1-50 / 191. |
| Positive control: | Jurkat cell lysate, MDA-MB-231 cell lysate, K-562 cell lysate, Hela cell lysate, MCF7 cell lysate, PANC-1 cell lysate, COS-1 cell lysate, HeLa. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB FC |
1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P84095 Human | A0A0D9QW62 Green monkey |
| Alternative names: | ARHG Ras homolog family member G Rho G Rho related GTP binding protein RhoG precursor Rho-related GTP-binding protein RhoG RHOG RHOG_HUMAN |
Images
|
Fig1:
Western blot analysis of RHOG on different lysates with Rabbit anti-RHOG antibody (HA721848) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: MDA-MB-231 cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: Hela cell lysate (20 µg/Lane) Lane 5: MCF7 cell lysate (20 µg/Lane) Lane 6: PANC-1 cell lysate (20 µg/Lane) Lane 7: COS-1 cell lysate (20 µg/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 30 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721848) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of HeLa cells labeling RHOG. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721848, 1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.