Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Monkey |
Applications: | WB, FC |
Clonality: | Monoclonal |
Clone number: | PSH02-70 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 21 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human RHOG aa 1-50 / 191. |
Positive control: | Jurkat cell lysate, MDA-MB-231 cell lysate, K-562 cell lysate, Hela cell lysate, MCF7 cell lysate, PANC-1 cell lysate, COS-1 cell lysate, HeLa. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
WB FC |
1:2,000 1:1,000 |
Uniprot #: | SwissProt: P84095 Human | A0A0D9QW62 Green monkey |
Alternative names: | ARHG Ras homolog family member G Rho G Rho related GTP binding protein RhoG precursor Rho-related GTP-binding protein RhoG RHOG RHOG_HUMAN |
Fig1:
Western blot analysis of RHOG on different lysates with Rabbit anti-RHOG antibody (HA721848) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: MDA-MB-231 cell lysate Lane 3: K-562 cell lysate Lane 4: Hela cell lysate Lane 5: MCF7 cell lysate Lane 6: PANC-1 cell lysate Lane 7: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721848) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Flow cytometric analysis of HeLa cells labeling RHOG. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721848, 1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |