RHOG Recombinant Rabbit Monoclonal Antibody [PSH02-70]
cat.: HA721848
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Monkey
Applications: WB, FC
Clonality: Monoclonal
Clone number: PSH02-70
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 21 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human RHOG aa 1-50 / 191.
Positive control: Jurkat cell lysate, MDA-MB-231 cell lysate, K-562 cell lysate, Hela cell lysate, MCF7 cell lysate, PANC-1 cell lysate, COS-1 cell lysate, HeLa.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  FC

1:2,000
1:1,000
Uniprot #: SwissProt: P84095 Human | A0A0D9QW62 Green monkey
Alternative names: ARHG Ras homolog family member G Rho G Rho related GTP binding protein RhoG precursor Rho-related GTP-binding protein RhoG RHOG RHOG_HUMAN
Images
HA721848_1.jpg Fig1: Western blot analysis of RHOG on different lysates with Rabbit anti-RHOG antibody (HA721848) at 1/2,000 dilution.

Lane 1: Jurkat cell lysate
Lane 2: MDA-MB-231 cell lysate
Lane 3: K-562 cell lysate
Lane 4: Hela cell lysate
Lane 5: MCF7 cell lysate
Lane 6: PANC-1 cell lysate
Lane 7: COS-1 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 21 kDa
Observed band size: 21 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721848) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721848_2.jpg Fig2: Flow cytometric analysis of HeLa cells labeling RHOG.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721848, 1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.