Cyclophilin 40 Recombinant Rabbit Monoclonal Antibody [PSH02-71]
cat.: HA721849
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB, IHC-P, FC, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH02-71 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Cyclophilin 40 aa 151-370 / 370. |
| Positive control: | HeLa cell lysate, 293T cell lysate, K-562 cell lysate, HepG2 cell lysate, MCF7 cell lysate, Jurkat cell lysate, COS-1 cell lysate, human liver tissue lysate, human kidney tissue, 293T. |
| Subcellular location: | Cytoplasm, Nucleus, nucleolus, nucleoplasm. |
| Recommended Dilutions:
WB IHC-P FC IF-Cell |
1:1,000 1:2,000 1:1,000 1:100 |
| Uniprot #: | SwissProt: Q08752 Human |
| Alternative names: | 40 kDa peptidyl prolyl cis trans isomerase 40 kDa peptidyl prolyl cis trans isomerase D 40 kDa peptidyl-prolyl cis-trans isomerase Cyclophilin D Cyclophilin related protein Cyclophilin-40 Cyclophilin-related protein Cyclophilin40 CyclophilinD CYP 40 Cyp D CYP-40 CYP40 CYPD MGC33096 Peptidyl Prolyl Isomerase D Peptidyl-prolyl cis-trans isomerase D Peptidylprolyl isomerase D PPIase PPIase D Ppid PPID_HUMAN Rotamase Rotamase D |
Images
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Fig1:
Western blot analysis of Cyclophilin 40 on different lysates with Rabbit anti-Cyclophilin 40 antibody (HA721849) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: K-562 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Jurkat cell lysate Lane 7: COS-1 cell lysate Lane 8: Human liver tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721849) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cyclophilin 40 antibody (HA721849) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721849) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of 293T cells labeling Cyclophilin 40 with Rabbit anti-Cyclophilin 40 antibody (HA721849) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cyclophilin 40 antibody (HA721849) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Flow cytometric analysis of 293T cells labeling Cyclophilin 40. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721849, 1:100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.