CD58 Recombinant Rabbit Monoclonal Antibody [PSH02-74]
cat.: HA721852
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH02-74 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD58 aa 29-215 (Extracellular). |
| Positive control: | HeLa cell lysate, Raji cell lysate, Jurkat cell lysate, K-562 cell lysate, HUVEC cell lysate, HT-29 cell lysate, TF-1 cell lysate, MCF7 cell lysate, human peripheral blood lymphocytes. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P19256 Human |
| Alternative names: | AG3 CD58 CD58 antigen (lymphocyte function associated antigen 3)1 CD58 antigen CD58 antigen, (lymphocyte function associated antigen 3) CD58 molecule FLJ23181 FLJ43722 LFA 3 LFA-3 LFA3 LFA3_HUMAN Lymphocyte Function Associated Antigen Type 3 Lymphocyte function-associated antigen 3 Surface glycoprotein LFA 3 Surface glycoprotein LFA-3 |
Images
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Fig1:
Western blot analysis of CD58 on different lysates with Rabbit anti-CD58 antibody (HA721852) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: Jurkat cell lysate Lane 4: K-562 cell lysate Lane 5: HUVEC cell lysate Lane 6: HT-29 cell lysate Lane 7: TF-1 cell lysate Lane 8: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 28 kDa Observed band size: 55 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721852) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of human peripheral blood lymphocytes labeling CD58. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA721852, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (black). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.