SAMD9 Recombinant Rabbit Monoclonal Antibody [PSH02-75]
cat.: HA721853
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH02-75 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 184 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SMAD9 aa 1-250 / 1,589. |
| Positive control: | A549 cell lysate, HeLa cell lysate, A431 cell lysate, Raji cell lysate, HEK-293 cell lysate, MCF7 cell lysate, HepG2 cell lysate, HCT 116 cell lysate, U-87 MG cell lysate, A431, human skin tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:100 1:200 1:1,000 |
| Uniprot #: | SwissProt: Q5K651 Human |
| Alternative names: | C7orf5 DRIF1 expressed in aggressive fibromatosis KIAA2004 NFTC OEF1 OEF2 SAM domain-containing protein 9 SAMD9 SAMD9_HUMAN sterile alpha motif domain containing 9 Sterile alpha motif domain-containing protein 9 |
Images
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Fig1:
Western blot analysis of SAMD9 on different lysates with Rabbit anti-SAMD9 antibody (HA721853) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HeLa cell lysate Lane 3: A431 cell lysate Lane 4: Raji cell lysate Lane 5: HEK-293 cell lysate Lane 6: MCF7 cell lysate Lane 7: HepG2 cell lysate Lane 8: HCT 116 cell lysate Lane 9: U-87 MG cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 184 kDa Observed band size: 184 kDa Exposure time: 2 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721853) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of A431 cells labeling SAMD9 with Rabbit anti-SAMD9 antibody (HA721853) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SAMD9 antibody (HA721853) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-SAMD9 antibody (HA721853) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721853) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of A431 cells labeling SAMD9. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721853, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Western blot analysis of SAMD9 on different lysates with Rabbit anti-SAMD9 antibody (HA721853) at 1/1,000 dilution. Lane 1: A431-si NT cell lysate Lane 2: A431-si SAMD9 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 184 kDa Observed band size: 184 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721853) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.