Heme Oxygenase 1(HO-1) Recombinant Rabbit Monoclonal Antibody [PSH02-76]
cat.: HA721854
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH02-76 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Heme oxygenase 1 aa 1-50 / 288. |
| Positive control: | HeLa cell lysate, A549 cell lysate, RAW264.7 cell lysate, A549, RAW264.7, human renal cell carcinoma tissue, mouse spleen tissue. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:5,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: P09601 Human | P14901 Mouse |
| Alternative names: | 32 kD bK286B10 D8Wsu38e heat shock protein 32 kD heat shock protein 32kD Heat shock protein Heme oxygenase (decycling) 1 Heme oxygenase 1 Hemox HMOX 1 Hmox Hmox1 HMOX1_HUMAN HO 1 HO HO-1 HO1 Hsp32 |
Images
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Fig1:
Western blot analysis of Heme Oxygenase 1(HO-1) on different lysates with Rabbit anti-Heme Oxygenase 1(HO-1) antibody (HA721854) at 1/5,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si Heme Oxygenase 1(HO-1) cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721854) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Heme Oxygenase 1(HO-1) on different lysates with Rabbit anti-Heme Oxygenase 1(HO-1) antibody (HA721854) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721854) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of A549 cells labeling Heme Oxygenase 1(HO-1) with Rabbit anti-Heme Oxygenase 1(HO-1) antibody (HA721854) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Heme Oxygenase 1(HO-1) antibody (HA721854) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunocytochemistry analysis of RAW264.7 cells labeling Heme Oxygenase 1(HO-1) with Rabbit anti-Heme Oxygenase 1(HO-1) antibody (HA721854) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Heme Oxygenase 1(HO-1) antibody (HA721854) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue with Rabbit anti-Heme Oxygenase 1(HO-1) antibody (HA721854) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721854) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Heme Oxygenase 1(HO-1) antibody (HA721854) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721854) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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