NSUN6 Recombinant Rabbit Monoclonal Antibody [PSH02-80]
cat.: HA721858
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH02-80 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NSUN6 aa 1-469 / 469. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, HepG2 cell lysate, K-562 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, rat pancreas tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q8TEA1 Human | Q7TS68 Mouse Entrez Gene: 307148 Rat |
| Alternative names: | 4933414E04Rik FLJ23743 NOL1/NOP2/Sun and PUA domain-containing protein 1 NOL1/NOP2/Sun domain family 6 NOL1/NOP2/Sun domain family member 6 NOP2 NOPD1 NSUN6 NSUN6_HUMAN nucleolar protein (NOL1/NOP2/sun) and PUA domains 1 Putative methyltransferase NSUN6 Sun domain family, member 6 |
Images
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Fig1:
Western blot analysis of NSUN6 on different lysates with Rabbit anti-NSUN6 antibody (HA721858) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: Jurkat cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Rat pancreas tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721858) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.