Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, FC |
Clonality: | Monoclonal |
Clone number: | PSH02-80 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 52 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human NSUN6 aa 1-469 / 469. |
Positive control: | HeLa cell lysate, HEK-293 cell lysate, HepG2 cell lysate, K-562 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, rat pancreas tissue lysate, HeLa, NIH/3T3. |
Subcellular location: | Cytoplasm. |
Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:100 1:1,000 |
Uniprot #: | SwissProt: Q8TEA1 Human | Q7TS68 Mouse Entrez Gene: 307148 Rat |
Alternative names: | 4933414E04Rik FLJ23743 NOL1/NOP2/Sun and PUA domain-containing protein 1 NOL1/NOP2/Sun domain family 6 NOL1/NOP2/Sun domain family member 6 NOP2 NOPD1 NSUN6 NSUN6_HUMAN nucleolar protein (NOL1/NOP2/sun) and PUA domains 1 Putative methyltransferase NSUN6 Sun domain family, member 6 |
Fig1:
Western blot analysis of NSUN6 on different lysates with Rabbit anti-NSUN6 antibody (HA721858) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: Jurkat cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Rat pancreas tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721858) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling NSUN6 with Rabbit anti-NSUN6 antibody (HA721858) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NSUN6 antibody (HA721858) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Fig3:
Immunocytochemistry analysis of NIH/3T3 cells labeling NSUN6 with Rabbit anti-NSUN6 antibody (HA721858) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NSUN6 antibody (HA721858) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Flow cytometric analysis of HeLa cells labeling NSUN6. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721858, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Flow cytometric analysis of NIH/3T3 cells labeling NSUN6. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721858, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |