FABP3 Recombinant Rabbit Monoclonal Antibody [JE53-37]
cat.: HA721859
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE53-37 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human FABP3 aa 1-50 / 133. |
| Positive control: | Mouse heart tissue lysate, rat heart tissue lysate, human heart tissue, mouse heart tissue, rat heart tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:100 |
| Uniprot #: | SwissProt: P05413 Human | P11404 Mouse | P07483 Rat |
| Alternative names: | 422 protein Cardiac FABP Cardiac Fatty Acid Binding Protein FABP 11 FABP 3 FABP11 FABP3 FABPH_HUMAN Fatty acid binding protein 11 Fatty acid binding protein 3 Fatty acid binding protein 3 muscle and heart Fatty acid binding protein 3 muscle and heart mammary derived growth inhibitor Fatty acid binding protein 3 muscle Fatty acid binding protein 3, muscle and heart (mammary derived growth inhibitor) Fatty acid binding protein 3, muscle Fatty acid binding protein heart Fatty acid binding protein, heart Fatty acid binding protein, muscle and heart Fatty acid binding protein, skeletal muscle Fatty acid-binding protein 3 Fatty acid-binding protein H FABP H-FABP heart Heart type fatty acid binding protein Heart-type fatty acid-binding protein M FABP M-FABP Mammary derived growth inhibitor Mammary-derived growth inhibitor MDGI Muscle fatty acid binding protein Muscle fatty acid-binding protein Mylein protein P2 homolog O FABP...... |
Images
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Fig1:
Western blot analysis of FABP3 on different lysates with Rabbit anti-FABP3 antibody (HA721859) at 1/2,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Rat heart tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721859) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-FABP3 antibody (HA721859) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721859) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-FABP3 antibody (HA721859) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721859) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-FABP3 antibody (HA721859) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721859) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.