CNOT7 Recombinant Rabbit Monoclonal Antibody [PSH02-81]
cat.: HA721861
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH02-81 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CNOT7 aa 1-285 / 285. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, NCCIT cell lysate, Jurkat cell lysate, THP-1 cell lysate, mouse brain tissue lysate, rat spleen tissue lysate, F9. |
| Subcellular location: | Nucleus, Cytoplasm, P-body, Cytoplasmic ribonucleoprotein granule. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q9UIV1 Human | Q60809 Mouse Entrez Gene: 306492 Rat |
| Alternative names: | BTG1 binding factor 1 BTG1-binding factor 1 CAF 1 CAF-1 CAF1 Carbon catabolite repressor protein (CCR4) associative factor 1 CCR4 associated factor 1 CCR4 NOT transcription complex subunit 7 CCR4-associated factor 1 CCR4-NOT transcription complex subunit 7 CNOT 7 Cnot7 CNOT7_HUMAN hCAF 1 hCAF1 |
Images
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Fig1:
Western blot analysis of CNOT7 on different lysates with Rabbit anti-CNOT7 antibody (HA721861) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: NCCIT cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: THP-1 cell lysate (20 µg/Lane) Lane 6: Mouse brain tissue lysate (40 µg/Lane) Lane 7: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721861) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of F9 cells labeling CNOT7 with Rabbit anti-CNOT7 antibody (HA721861) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CNOT7 antibody (HA721861) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of F9 cells labeling CNOT7. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721861, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.