MYH2 Recombinant Rabbit Monoclonal Antibody [JE53-30]
cat.: HA721862
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE53-30 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 223 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MYH2 aa 30-129 / 1,941. |
| Positive control: | Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:5,000 |
| Uniprot #: | SwissProt: Q9UKX2 Human | Q5SX41 Mouse | Q07443 Rat |
| Alternative names: | adult 2 Fast 2a myosin heavy chain IBM3 Inclusion body myopathy 3, autosomal dominant MYH2 MYH2_HUMAN MYH2A MYHas8 MyHC IIa MyHC-2a MyHC-IIa MYHSA2 Myosin heavy chain 2 Myosin heavy chain 2a Myosin heavy chain Myosin heavy chain IIa Myosin heavy chain skeletal muscle adult 2 Myosin heavy polypeptide 2 skeletal muscle adult Myosin-2 MYPOP skeletal muscle Type IIA myosin heavy chain |
Images
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Fig1:
Western blot analysis of MYH2 on different lysates with Rabbit anti-MYH2 antibody (HA721862) at 1/1,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Rat skeletal muscle tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 223 kDa Observed band size: 223 kDa Exposure time: 2 minutes 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721862) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-MYH2 antibody (HA721862) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721862) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-MYH2 antibody (HA721862) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721862) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-MYH2 antibody (HA721862) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721862) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Rabbit anti-MYH2 antibody (HA721862) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721862) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.