Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) Recombinant Rabbit Monoclonal Antibody [JE45-17]
cat.: HA721864
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE45-17 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Thr197 of PKA C-gamma. |
| Positive control: | NIH/3T3 cell lysate, C6 cell lysate, HeLa cell lysate, HeLa serum starved overnight then treated with 100nM Calyculin A for 1 hour cell lysate. |
| Subcellular location: | Cytoplasm, Cell membrane, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P17612 Human | P22694 Human | P22612 Human | P05132 Mouse | P27791 Rat |
| Alternative names: | cAMP dependent protein kinase catalytic subunit alpha cAMP dependent protein kinase catalytic subunit beta cAMP dependent protein kinase catalytic subunit gamma PKA C alpha PKA C beta PKA C gamma PKACA PRKACA PRKACB PRKACG |
Images
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Fig1:
Western blot analysis of Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) on different lysates with Rabbit anti-Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) antibody (HA721864) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: C6 cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa serum starved overnight then treated with 100nM Calyculin A for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721864) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.