Phospho-GluR1 (AMPA subtype) (S831) Recombinant Rabbit Monoclonal Antibody [JE44-96]
cat.: HA721865
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE44-96
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 102 kDa
Isotype: IgG
Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser831 of Glutamate Receptor 1
Positive control: Mouse brain tissue lysate, human brain tissue lysate.
Subcellular location: Cell membrane, Endoplasmic reticulum membrane, Cell junction, Cell projection.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P42261 Human | P23818 Mouse | P19490 Rat
Alternative names: GLUR 1 GLUR A AMPA 1 AMPA selective glutamate receptor 1 AMPA-selective glutamate receptor 1 GluA1 GLUH1 GluR K1 GluR-1 GluR-A GluR-K1 GLUR1 GLURA GluRK1 Glutamate receptor 1 Glutamate receptor ionotropic AMPA 1 Glutamate receptor ionotropic Glutamate receptor, ionotropic, AMPA 1 Gria1 GRIA1_HUMAN HBGR1 MGC133252 OTTHUMP00000160643 OTTHUMP00000165781 OTTHUMP00000224241 OTTHUMP00000224242 OTTHUMP00000224243
Images
HA721865_1.jpg Fig1: Western blot analysis of Phospho-GluR1 (AMPA subtype) (S831) on different lysates with Rabbit anti-Phospho-GluR1 (AMPA subtype) (S831) antibody (HA721865) at 1/1,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Human brain tissue lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 102 kDa
Observed band size: 110 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721865) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.