Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE44-96 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 102 kDa |
Isotype: | IgG |
Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Ser831 of Glutamate Receptor 1 |
Positive control: | Mouse brain tissue lysate, human brain tissue lysate. |
Subcellular location: | Cell membrane, Endoplasmic reticulum membrane, Cell junction, Cell projection. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: P42261 Human | P23818 Mouse | P19490 Rat |
Alternative names: | GLUR 1 GLUR A AMPA 1 AMPA selective glutamate receptor 1 AMPA-selective glutamate receptor 1 GluA1 GLUH1 GluR K1 GluR-1 GluR-A GluR-K1 GLUR1 GLURA GluRK1 Glutamate receptor 1 Glutamate receptor ionotropic AMPA 1 Glutamate receptor ionotropic Glutamate receptor, ionotropic, AMPA 1 Gria1 GRIA1_HUMAN HBGR1 MGC133252 OTTHUMP00000160643 OTTHUMP00000165781 OTTHUMP00000224241 OTTHUMP00000224242 OTTHUMP00000224243 |
Fig1:
Western blot analysis of Phospho-GluR1 (AMPA subtype) (S831) on different lysates with Rabbit anti-Phospho-GluR1 (AMPA subtype) (S831) antibody (HA721865) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 102 kDa Observed band size: 110 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721865) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |