Phospho-Glucose Transporter GLUT4 (S488) Recombinant Rabbit Monoclonal Antibody [JE43-23]
cat.: HA721866
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Human, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE43-23 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Ser488 of Glucose Transporter GLUT4. |
| Positive control: | Mouse heart tissue lysates (no heat). |
| Subcellular location: | Cell membrane, Endomembrane system, Cytoplasm, perinuclear region. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P14672 Human | P14142 Mouse | P19357 Rat |
| Alternative names: | Glucose transporter GLUT 4 Glucose transporter type 4 Glucose transporter type 4 insulin responsive GLUT 4 GLUT-4 GLUT4 GTR4_HUMAN Insulin responsive glucose transporter type 4 insulin-responsive kug SLC 2A4 SLC2A4 solute carrier family 2 (facilitated glucose transporter) member 4 Solute carrier family 2 member 4 Solute carrier family 2, facilitated glucose transporter member 4 |
Images
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Fig1:
Western blot analysis of Phospho-Glucose Transporter GLUT4 (S488) on mouse heart tissue lysates (no heat) with Rabbit anti-Phospho-Glucose Transporter GLUT4 (S488) antibody (HA721866) at 1/1,000 dilution. Notice: no heat means the lysate is not boiled. Lysates/proteins at 30 µg/Lane. Predicted band size: 55 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721866) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.