Phospho-Glucose Transporter GLUT4 (S488) Recombinant Rabbit Monoclonal Antibody [JE43-23]
cat.: HA721866
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse, Human, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE43-23
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 55 kDa
Isotype: IgG
Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser488 of Glucose Transporter GLUT4.
Positive control: Mouse heart tissue lysates (no heat).
Subcellular location: Cell membrane, Endomembrane system, Cytoplasm, perinuclear region.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P14672 Human | P14142 Mouse | P19357 Rat
Alternative names: Glucose transporter GLUT 4 Glucose transporter type 4 Glucose transporter type 4 insulin responsive GLUT 4 GLUT-4 GLUT4 GTR4_HUMAN Insulin responsive glucose transporter type 4 insulin-responsive kug SLC 2A4 SLC2A4 solute carrier family 2 (facilitated glucose transporter) member 4 Solute carrier family 2 member 4 Solute carrier family 2, facilitated glucose transporter member 4
Images
HA721866_1.jpg Fig1: Western blot analysis of Phospho-Glucose Transporter GLUT4 (S488) on mouse heart tissue lysates (no heat) with Rabbit anti-Phospho-Glucose Transporter GLUT4 (S488) antibody (HA721866) at 1/1,000 dilution.

Lysates/proteins at 30 µg/Lane.

Predicted band size: 55 kDa
Observed band size: 50 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721866) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.