APOE4 Recombinant Rabbit Monoclonal Antibody [PSH02-85]
cat.: HA721867
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH02-85 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Apolipoprotein E aa 101-150 / 317. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P02649 Human |
| Alternative names: | AD2 Apo-E APOE APOE_HUMAN APOEA Apolipoprotein E ApolipoproteinE Apoprotein LDLCQ5 LPG Apolipoprotein-E Apolipoprotein E4 Apolipoprotein-E4 |
Images
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Fig1:
Western blot analysis of APOE4 on different lysates with Rabbit anti-APOE4 antibody (HA721867) at 1/2,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-APOE2 cell lysate Lane 3: 293T-OE-APOE3 cell lysate Lane 4: 293T-OE-APOE4 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 2 minutes 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721867) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of APOE4 on different lysates with Rabbit anti-APOE4 antibody (HA721867) at 1/2,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD APOE4 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 30 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721867) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.