xCT/SLC7A11 Recombinant Rabbit Monoclonal Antibody [JE31-93]
cat.: HA721868
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JE31-93 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human xCT aa 1-50 / 501. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, 293T cell lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate, PC-12 cell lysates. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IP |
1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q9UPY5 Human | Q9WTR6 Mouse Entrez Gene: 310392 Rat |
| Alternative names: | Amino acid transport system xc xCT Amino acid transport system xc- Calcium channel blocker resistance protein CCBR1 Calcium channel blocker resistance protein CCBR1 CCBR1 Cysteine/glutamate transporter Cystine/glutamate transporter OTTHUMP00000164578 SLC7A11 Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 solute carrier family 7 Solute carrier family 7 member 11 Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN xCT XCT_HUMAN |
Images
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Fig1:
Western blot analysis of xCT/SLC7A11 on different lysates with Rabbit anti-xCT/SLC7A11 antibody (HA721868) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Mouse brain tissue lysate (40 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Lane 7: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721868) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of xCT/SLC7A11 on PC-12 cell lysates with Rabbit anti-xCT/SLC7A11 antibody (HA721868) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721868) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
xCT/SLC7A11 was immunoprecipitated in 0.2mg HeLa cell lysate with HA721868 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA721868 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA721868 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA721868 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.