NDUFV2 Recombinant Rabbit Monoclonal Antibody [PSH02-86]
cat.: HA721869
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell
Clonality: Monoclonal
Clone number: PSH02-86
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 27 kDa
Isotype: IgG
Immunogen: Recombinant protein within human NDUFV2 aa 1-249 / 249.
Positive control: A549 cell lysate, HeLa cell lysate, Jurkat cell lysate, Ramos cell lysate, Raji cell lysate, K-562 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse heart tissue lysate, rat heart tissue lysate, human kidney tissue lysate, mouse kidney tissue lysate, RAW264.7, human heart tissue, human kidney tissue, mouse heart tissue, mouse kidney tissue, rat heart tissue, rat kidney tissue.
Subcellular location: Mitochondrion inner membrane.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
1:2,000
1:2,000-1:5,000
1:2,000
Uniprot #: SwissProt: P19404 Human | Q9D6J6 Mouse | P19234 Rat
Alternative names: 24kDa subunit of Complex I CI-24k complex I 24kDa subunit complex I, mitochondrial respiratory 2 mitochondrial NADH dehydrogenase (ubiquinone) flavoprotein 2 NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa NADH dehydrogenase [ubiquinone] flavoprotein 2 NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial NADH ubiquinone oxidoreductase 24 kDa subunit NADH-ubiquinone oxidoreductase 24 kDa subunit NADH-ubiquinone oxidoreductase flavoprotein 2 NDUFV2 NDUV2_HUMAN nuclear-encoded mitochondrial NADH-ubiquinone reductase 24Kd subunit Ubiquinoneflavoprotein 2 Ubiquinoneflavoprotein 2, mitochondrial precursor
Images
HA721869_1.jpg Fig1: Western blot analysis of NDUFV2 on different lysates with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.

Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: Ramos cell lysate (20 µg/Lane)
Lane 5: Raji cell lysate (20 µg/Lane)
Lane 6: K-562 cell lysate (20 µg/Lane)
Lane 7: A431 cell lysate (20 µg/Lane)
Lane 8: NIH/3T3 cell lysate (20 µg/Lane)
Lane 9: RAW264.7 cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)
Lane 11: Mouse heart tissue lysate (40 µg/Lane)
Lane 12: Rat heart tissue lysate (40 µg/Lane)
Lane 13: Human kidney tissue lysate (40 µg/Lane)
Lane 14: Mouse kidney tissue lysate (40 µg/Lane)

Predicted band size: 27 kDa
Observed band size: 24 kDa

Exposure time: 1 minute 59 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721869_2.jpg Fig2: Immunocytochemistry analysis of RAW264.7 cells labeling NDUFV2 with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
HA721869_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721869_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFV2 antibody (HA721869) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721869) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721869_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721869_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721869_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721869_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721869) at 1/2,000 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721869_9.jpg Fig9: Western blot analysis of NDUFV2 on different lysates with Rabbit anti-NDUFV2 antibody (HA721869) at 1/2,000 dilution.

Lane 1: A549-si NT cell lysate
Lane 2: A549-si NDUFV2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 27 kDa
Observed band size: 24 kDa

Exposure time: 16 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721869) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.