CDT2 Recombinant Rabbit Monoclonal Antibody [JE58-01]
cat.: HA721871
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE58-01 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 79 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CDT2 aa 1-200 / 730. |
| Positive control: | HeLa cell lysate, 293T cell lysate, K-562 cell lysate, Jurkat cell lysate, mouse spleen tissue lysate, HeLa. |
| Subcellular location: | Nucleus, Nucleus membrane, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Chromosome. |
| Recommended Dilutions:
WB FC |
1:2,000 1:1,000 |
| Uniprot #: | SwissProt: Q9NZJ0 Human | Q3TLR7 Mouse |
| Alternative names: | Lethal(2) denticleless protein homolog CDW1 DCAF2 DDB1 and CUL4 associated factor 2 Ddb1- and Cul4-associated factor 2 Denticleless homolog Denticleless homolog (Drosophila) Denticleless protein homolog Dtl DTL_HUMAN L2DTL Lethal(2) denticleless protein homolog RA regulated nuclear matrix associated protein RAMP Retinoic acid regulated nuclear matrix associated protein Retinoic acid-regulated nuclear matrix-associated protein |
Images
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Fig1:
Western blot analysis of CDT2 on different lysates with Rabbit anti-CDT2 antibody (HA721871) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: Mouse spleen tissue lysate (40 µg/Lane) Predicted band size: 79 kDa Observed band size: 79 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721871) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of HeLa cells labeling CDT2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721871, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.