HA721872

UQCRC2 Recombinant Rabbit Monoclonal Antibody [JE32-56]

cat.: HA721872

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P, FC
Clonality:	Monoclonal
Clone number:	JE32-56

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 48 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human UQCRC2 aa 285-384 / 453.
Positive control:	HepG2 cell lysate, HEK-293 cell lysate, Jurkat cell lysate, C6 cell lysate, HepG2, human liver tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Subcellular location:	Mitochondrion inner membrane.
Recommended Dilutions: WB IF-Cell IHC-P FC	1:2,000 1:100 1:500-1:2,000 1:1,000
Uniprot #:	SwissProt: P22695 Human \| Q9DB77 Mouse \| P32551 Rat
Alternative names:	Complex III subunit 2 Core protein II Cytochrome b c1 complex subunit 2 mitochondrial Cytochrome b-c1 complex subunit 2 EC 1.10.2.2 MC3DN5 mitochondrial QCR2 QCR2_HUMAN Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial Ubiquinol cytochrome c reductase core protein II Ubiquinol-cytochrome-c reductase complex core protein 2 UQCR2 Uqcrc2

Images

Fig1: Western blot analysis of UQCRC2 on different lysates with Rabbit anti-UQCRC2 antibody (HA721872) at 1/2,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: C6 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 48 kDa
Observed band size: 48 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721872) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Immunocytochemistry analysis of HepG2 cells labeling UQCRC2 with Rabbit anti-UQCRC2 antibody (HA721872) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-UQCRC2 antibody (HA721872) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

	Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-UQCRC2 antibody (HA721872) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721872) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-UQCRC2 antibody (HA721872) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721872) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-UQCRC2 antibody (HA721872) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721872) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-UQCRC2 antibody (HA721872) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721872) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Flow cytometric analysis of HepG2 cells labeling UQCRC2.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721872, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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