CD53 Recombinant Rabbit Monoclonal Antibody [JE60-38]
cat.: HA721873
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE60-38 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD53 aa 170-219 / 219. |
| Positive control: | Jurkat cell lysate, Raji cell lysate, Ramos cell lysate, Jurkat, human tonsil tissue. |
| Subcellular location: | Cell membrane, Cell junction, Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000 1:100 1:2,000 |
| Uniprot #: | SwissProt: P19397 Human |
| Alternative names: | AI323659 Antigen MOX44 identified by monoclonal MRC-OX44 CD 53 CD53 CD53 antigen CD53 antigen, isoform CRA_b CD53 glycoprotein Cd53 molecule CD53 tetraspan antigen CD53_HUMAN Cell surface glycoprotein CD53 Leukocyte antigen MRC OX-44 Leukocyte surface antigen CD53 MOX 44 MOX44 OTTHUMP00000059505 Ox-44 OX44 RATOX44 Tetraspanin 25 Tetraspanin-25 Tetraspanin25 Transmembrane glycoprotein TSPAN 25 Tspan-25 TSPAN25 |
Images
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Fig1:
Western blot analysis of CD53 on different lysates with Rabbit anti-CD53 antibody (HA721873) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Raji cell lysate Lane 3: Ramos cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 35-42 kDa Exposure time: 1 minute 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721873) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of Jurkat cells labeling CD53 with Rabbit anti-CD53 antibody (HA721873) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD53 antibody (HA721873) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD53 antibody (HA721873) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721873) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.