SYVN1 Recombinant Rabbit Monoclonal Antibody [JE44-67]
cat.: HA721874
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE44-67 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SYVN1 aa 150-250. |
| Positive control: | HEK-293 cell lysate, HepG2 cell lysate, Raji cell lysate, HCT 116 cell lysate, HeLa cell lysate, HeLa. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q86TM6 Human |
| Alternative names: | 1200010C09Rik DER3 E3 ubiquitin-protein ligase synoviolin HMG coA reductase degradation 1 homolog HRD1 KIAA1810 MGC40372 OTTHUMP00000230429 OTTHUMP00000230430 OTTHUMP00000230431 OTTHUMP00000230432 Synovial apoptosis inhibitor 1 Synovial apoptosis inhibitor 1, synoviolin Synoviolin 1 Synoviolin 1 isoform b SYNOVIOLIN SYVN1 SYVN1_HUMAN |
Images
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Fig1:
Western blot analysis of SYVN1 on different lysates with Rabbit anti-SYVN1 antibody (HA721874) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HepG2 cell lysate Lane 3: Raji cell lysate Lane 4: HCT 116 cell lysate Lane 5: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721874) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling SYVN1 with Rabbit anti-SYVN1 antibody (HA721874) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SYVN1 antibody (HA721874) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Flow cytometric analysis of HeLa cells labeling SYVN1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721874, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.