GALT Recombinant Rabbit Monoclonal Antibody [PSH02-88]
cat.: HA721876
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH02-88 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GALT aa 1-379 / 379. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, A549 cell lysate, K-562 cell lysate, THP-1 cell lysate, Jurkat cell lysate, SiHa cell lysate, SH-SY5Y cell lysate, mouse liver tissue lysate. |
| Subcellular location: | Cytosol, Golgi apparatus, cytoplasm. |
| Recommended Dilutions:
WB |
1:2,000-1:5,000 |
| Uniprot #: | SwissProt: P07902 Human | Q03249 Mouse |
| Alternative names: | Gal 1 P uridylyltransferase Gal-1-P uridylyltransferase Galactose 1 phosphate uridyl transferase Galactose 1 phosphate uridylyltransferase Galactose-1-phosphate uridylyltransferase GALT GALT_HUMAN UDP glucose hexose 1 phosphate uridylyltransferase UDP-glucose--hexose-1-phosphate uridylyltransferase |
Images
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Fig1:
Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (HA721876) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: A549 cell lysate (15 µg/Lane) Lane 4: K-562 cell lysate (15 µg/Lane) Lane 5: THP-1 cell lysate (15 µg/Lane) Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: Lane 1-5 (left): 1 minute 34 seconds; ECL: K1801; Exposure time: Lane 1-5 (right): 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721876) at 1/5,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (HA721876) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-GALT KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721876) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (HA721876) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: THP-1 cell lysate (20 µg/Lane) Lane 6: Jurkat cell lysate (20 µg/Lane) Lane 7: SiHa cell lysate (20 µg/Lane) Lane 8: SH-SY5Y cell lysate (20 µg/Lane) Lane 9: Mouse liver tissue lysate (40 µg/Lane) Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 43 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721876) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.