NFIB / NF1B2 Recombinant Rabbit Monoclonal Antibody [JE35-21]
cat.: HA721879
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell
Clonality: Monoclonal
Clone number: JE35-21
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 47 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human NFIB / NF1B2 aa 371-420 / 420.
Positive control: SH-SY5Y cell lysate, C6 cell lysate, human lung tissue lysate, mouse lung tissue lysate, HeLa, human breast tissue, mouse liver tissue, mouse lung tissue, rat liver tissue, rat lung tissue.
Subcellular location: Nucleus
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
1:5,000-1:10,000
1:500-1:2,000
1:100
Uniprot #: SwissProt: O00712 Human | P97863 Mouse
Entrez Gene: 29227 Rat
Alternative names: CCAAT Box Binding Transcription Factor CCAAT-box-binding transcription factor CTF HMGIC/NFIB NF-I/B NF1-B NF1B NF1B2 NFI-B NFI-RED Nfib NFIB_HUMAN NFIB2 NFIB3 Nuclear factor 1 B-type Nuclear factor 1/B Nuclear Factor 1B Nuclear Factor I B Nuclear factor I/B TGGCA Binding Protein TGGCA-binding protein TRANSCRIPTION FACTOR NFIB
Images
HA721879_1.jpg Fig1: Western blot analysis of NFIB / NF1B2 on different lysates with Rabbit anti-NFIB / NF1B2 antibody (HA721879) at 1/10,000 dilution.

Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: C6 cell lysate (20 µg/Lane)
Lane 3: Human lung tissue lysate (40 µg/Lane)
Lane 4: Mouse lung tissue lysate (40 µg/Lane)

Predicted band size: 47 kDa
Observed band size: 47 kDa

Exposure time: 1 minute 2 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721879) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721879_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling NFIB / NF1B2 with Rabbit anti-NFIB / NF1B2 antibody (HA721879) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NFIB / NF1B2 antibody (HA721879) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
HA721879_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-NFIB / NF1B2 antibody (HA721879) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721879) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721879_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-NFIB / NF1B2 antibody (HA721879) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721879) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721879_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-NFIB / NF1B2 antibody (HA721879) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721879) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721879_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-NFIB / NF1B2 antibody (HA721879) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721879) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721879_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-NFIB / NF1B2 antibody (HA721879) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721879) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.