RNA polymerase beta Recombinant Rabbit Monoclonal Antibody [JE32-42]
cat.: HA721880
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Escherichia coli |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE32-42 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 150 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within E.coli RNA polymerase beta aa 980-1,342 / 1,342. |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P0A8V2 Escherichia coli Entrez Gene: 948488 Escherichia coli |
| Alternative names: | groN nitB rif RNA pol beta RNA polymerase subunit beta RNA polymerase, beta subunit RNAP subunit beta ron rpoB stl stv tabD Transcriptase subunit beta |
Images
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Fig1:
Western blot analysis of RNA polymerase beta on different lysates with Rabbit anti-RNA polymerase beta antibody (HA721880) at 1/2,000 dilution. Lane 1: E.coli cell lysate Lane 2: E.coli cell lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 10 µg/Lane. Predicted band size: 150 kDa Observed band size: 150 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721880) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.