RNA polymerase beta Recombinant Rabbit Monoclonal Antibody [JE32-42]
cat.: HA721880
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Escherichia coli
Applications: WB
Clonality: Monoclonal
Clone number: JE32-42
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 150 kDa
Isotype: IgG
Immunogen: Recombinant protein within E.coli RNA polymerase beta aa 980-1,342 / 1,342.
Subcellular location: Cytoplasm
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: P0A8V2 Escherichia coli
Entrez Gene: 948488 Escherichia coli
Alternative names: groN nitB rif RNA pol beta RNA polymerase subunit beta RNA polymerase, beta subunit RNAP subunit beta ron rpoB stl stv tabD Transcriptase subunit beta
Images
HA721880_1.jpg Fig1: Western blot analysis of RNA polymerase beta on different lysates with Rabbit anti-RNA polymerase beta antibody (HA721880) at 1/2,000 dilution.

Lane 1: E.coli cell lysate
Lane 2: E.coli cell lysate (no heat)

Lysates/proteins at 10 µg/Lane.

Predicted band size: 150 kDa
Observed band size: 150 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721880) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.