Otx1 Recombinant Rabbit Monoclonal Antibody [JE42-10]
cat.: HA721881
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE42-10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Otx1 aa 1-100 / 354. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate. |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P32242 Human | Q5SS54 Mouse | Q63410 Rat |
| Alternative names: | FLJ38361 Homeobox protein OTX 1 Homeobox protein OTX1 MGC15736 Orthodenticle 1 Orthodenticle homeobox 1 Orthodenticle homolog 1 Orthodenticle1 Otx 1 otx1 OTX1_HUMAN |
Images
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Fig1:
Western blot analysis of Otx1 on different lysates with Rabbit anti-Otx1 antibody (HA721881) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721881) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.