Ras Recombinant Rabbit Monoclonal Antibody [JE44-13]
cat.: HA721883
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE44-13 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Ras aa 1-50 / 189. |
| Positive control: | HEK-293 cell lysate, Jurkat cell lysate, SH-SY5Y cell lysate, MCF7 cell lysate, A431 cell lysate, A375 cell lysate, C6 cell lysate, NIH/3T3 cell lysate, rat spleen tissue lysate, mouse spleen tissue lysate, MCF7. |
| Subcellular location: | Cell membrane, Golgi apparatus membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000-1:2,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: P01111 Human | P01112 Human | P01116 Human | P08556 Mouse | P32883 Mouse | Q61411 Mouse | P08644 Rat | P20171 Rat | Q04970 Rat |
| Alternative names: | C BAS/HAS C HA RAS1 C-BAS/HAS c-H-ras C-HA-RAS1 CTLO GTPase HRas GTPase KRas GTPase NRas H ras H RASIDX H-Ras-1 H-RASIDX Ha-Ras HAMSV HRAS HRAS1 K ras K RAS2A K RAS2B K RAS4A K RAS4B K-RAS KRAS KRAS1 KRAS2 N-RAS N-terminally processed NRAS NRAS1 p21ras RASH_HUMAN RASH1 RASK2 Transforming protein p21 v Ha ras Harvey rat sarcoma viral oncogene homolog v Ki ras2 Kirsten rat sarcoma viral oncogene homolog v ras neuroblastoma RAS viral oncogene homolog |
Images
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Fig1:
Western blot analysis of Ras on different lysates with Rabbit anti-Ras antibody (HA721883) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: SH-SY5Y cell lysate (20 µg/Lane) Lane 4: MCF7 cell lysate (20 µg/Lane) Lane 5: A431 cell lysate (20 µg/Lane) Lane 6: A375 cell lysate (20 µg/Lane) Lane 7: C6 cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: Rat spleen tissue lysate (40 µg/Lane) Lane 10: Mouse spleen tissue lysate (40 µg/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721883) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of MCF7 cells labeling Ras with Rabbit anti-Ras antibody (HA721883) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ras antibody (HA721883) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Flow cytometric analysis of MCF7 cells labeling Ras. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721883, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.