GRK6 Recombinant Rabbit Monoclonal Antibody [JE63-06]
cat.: HA721884
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE63-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GRK6 aa 71-120 / 576. |
| Positive control: | HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, MCF7 cell lysate, K-562 cell lysate, A549 cell lysate, 293T. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P43250 Human |
| Alternative names: | FLJ32135 G protein coupled receptor kinase 6 G protein coupled receptor kinase GRK6 G protein-coupled receptor kinase 6 G protein-coupled receptor kinase GRK6 Gprk6 Grk6 GRK6_HUMAN |
Images
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Fig1:
Western blot analysis of GRK6 on different lysates with Rabbit anti-GRK6 antibody (HA721884) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si GRK6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721884) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of GRK6 on different lysates with Rabbit anti-GRK6 antibody (HA721884) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: K-562 cell lysate Lane 6: A549 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721884) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Flow cytometric analysis of 293T cells labeling GRK6. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721884, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.