Bag1 Recombinant Rabbit Monoclonal Antibody [JE63-22]
cat.: HA721885
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE63-22 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Bag1 aa 111-160 / 345. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, rat testis tissue lysate, mouse testis tissue lysate, human breast cancer tissue, human prostate cancer tissue, HeLa. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:1,000 1:500 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q99933 Human | Q60739 Mouse | B0K019 Rat |
| Alternative names: | Bag 1 BAG family molecular chaperone regulator 1 BAG-1 BAG1 BAG1_HUMAN BCL 2 Associated Athanogene 1 BCL 2 associated athanogene BCL 2 binding athanogene 1 Bcl-2-associated athanogene 1 BCL2 associated athanogene 1 BCL2 associated athanogene Glucocorticoid receptor-associated protein RAP46 HAP1 Haptoglobin RAP 46 RAP46 |
Images
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Fig1:
Western blot analysis of Bag1 on different lysates with Rabbit anti-Bag1 antibody (HA721885) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: HEK-293 cell lysate (20 µg/Lane) Lane 4: MCF7 cell lysate (20 µg/Lane) Lane 5: RAW264.7 cell lysate (20 µg/Lane) Lane 6: Rat testis tissue lysate (40 µg/Lane) Lane 7: Mouse testis tissue lysate (40 µg/Lane) Predicted band size: 38 kDa Observed band size: 35/46/52 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721885) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Bag1 antibody (HA721885) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721885) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-Bag1 antibody (HA721885) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721885) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of HeLa cells labeling Bag1 with Rabbit anti-Bag1 antibody (HA721885) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Bag1 antibody (HA721885) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Flow cytometric analysis of HeLa cells labeling Bag1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721885, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.