CRTC3 Recombinant Rabbit Monoclonal Antibody [JE63-18]
cat.: HA721887
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Monkey
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JE63-18
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 67 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CRTC3 aa 570-219 / 619.
Positive control: HeLa cell lysate, TT cell lysate, Jurkat cell lysate, HDLM-2 cell lysate, HEK-293 cell lysate, COS-1 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, human brain tissue, human hodgkin lymphoma tissue, human lung tissue, human tonsil tissue, TT, RAW264.7, PC-12.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:2,000
1:500
1:1,000
Uniprot #: SwissProt: Q6UUV7 Human | Q91X84 Mouse
Entrez Gene: 365297 Rat
Alternative names: CREB regulated transcription coactivator 3 CREB-regulated transcription coactivator 3 CRTC 3 CRTC3 CRTC3_HUMAN FLJ21868 TORC 3 TORC-3 TORC3 Transducer of CREB protein 3 Transducer of regulated cAMP response element binding protein (CREB) 3 Transducer of regulated cAMP response element binding protein 3 Transducer of regulated cAMP response element-binding protein 3 Transducer of regulated CREB protein 3
Images
HA721887_1.jpg Fig1: Western blot analysis of CRTC3 on different lysates with Rabbit anti-CRTC3 antibody (HA721887) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: TT cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HDLM-2 cell lysate
Lane 5: HEK-293 cell lysate
Lane 6: COS-1 cell lysate
Lane 7: RAW264.7 cell lysate
Lane 8: NIH/3T3 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: C6 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 67 kDa
Observed band size: 75 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721887) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721887_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-CRTC3 antibody (HA721887) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721887) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721887_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human hodgkin lymphoma tissue with Rabbit anti-CRTC3 antibody (HA721887) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721887) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721887_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-CRTC3 antibody (HA721887) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721887) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721887_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CRTC3 antibody (HA721887) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721887) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721887_6.jpg Fig6: Flow cytometric analysis of TT cells labeling CRTC3.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721887, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA721887_7.jpg Fig7: Flow cytometric analysis of RAW264.7 cells labeling CRTC3.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721887, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA721887_8.jpg Fig8: Flow cytometric analysis of PC-12 cells labeling CRTC3.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721887, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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