Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE36-22 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 163 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human FANCA aa 1-150 / 1,455. |
Positive control: | HeLa cell lysate, HEK-293 cell lysate, A431 cell lysate, Jurkat cell lysate, HCT 116 cell lysate, HT-29 cell lysate, MCF7 cell lysate, K-562 cell lysate, Daudi cell lysate. |
Subcellular location: | Nucleus, Cytoplasm. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: O15360 Human |
Alternative names: | FA 1 FA FA H FA1 FAA FACA FAH Fanca FANCA_HUMAN FANCH Fanconi anemia complementation group A Fanconi anemia complementation group H Fanconi anemia group A protein Fanconi anemia type 1 MGC75158 Protein FACA |
Fig1:
Western blot analysis of FANCA on different lysates with Rabbit anti-FANCA antibody (HA721889) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lane 4: Jurkat cell lysate Lane 5: HCT 116 cell lysate Lane 6: HT-29 cell lysate Lane 7: MCF7 cell lysate Lane 8: K-562 cell lysate Lane 9: Daudi cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 163 kDa Observed band size: 163 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721889) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |