FANCA Recombinant Rabbit Monoclonal Antibody [JE36-22]
cat.: HA721889
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE36-22
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 163 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human FANCA aa 1-150 / 1,455.
Positive control: HeLa cell lysate, HEK-293 cell lysate, A431 cell lysate, Jurkat cell lysate, HCT 116 cell lysate, HT-29 cell lysate, MCF7 cell lysate, K-562 cell lysate, Daudi cell lysate.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: O15360 Human
Alternative names: FA 1 FA FA H FA1 FAA FACA FAH Fanca FANCA_HUMAN FANCH Fanconi anemia complementation group A Fanconi anemia complementation group H Fanconi anemia group A protein Fanconi anemia type 1 MGC75158 Protein FACA
Images
HA721889_1.jpg Fig1: Western blot analysis of FANCA on different lysates with Rabbit anti-FANCA antibody (HA721889) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: A431 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HCT 116 cell lysate
Lane 6: HT-29 cell lysate
Lane 7: MCF7 cell lysate
Lane 8: K-562 cell lysate
Lane 9: Daudi cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 163 kDa
Observed band size: 163 kDa

Exposure time: 1 minute 59 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721889) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.