FANCA Recombinant Rabbit Monoclonal Antibody [JE36-22]
cat.: HA721889
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE36-22 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 163 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FANCA aa 1-150 / 1,455. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, A431 cell lysate, Jurkat cell lysate, HCT 116 cell lysate, HT-29 cell lysate, MCF7 cell lysate, K-562 cell lysate, Daudi cell lysate. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O15360 Human |
| Alternative names: | FA 1 FA FA H FA1 FAA FACA FAH Fanca FANCA_HUMAN FANCH Fanconi anemia complementation group A Fanconi anemia complementation group H Fanconi anemia group A protein Fanconi anemia type 1 MGC75158 Protein FACA |
Images
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Fig1:
Western blot analysis of FANCA on different lysates with Rabbit anti-FANCA antibody (HA721889) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lane 4: Jurkat cell lysate Lane 5: HCT 116 cell lysate Lane 6: HT-29 cell lysate Lane 7: MCF7 cell lysate Lane 8: K-562 cell lysate Lane 9: Daudi cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 163 kDa Observed band size: 163 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721889) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.