RAC3 Recombinant Rabbit Monoclonal Antibody [JE56-48]
cat.: HA721890
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE56-48 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RAC3 aa 71-170 / 192. |
| Positive control: | HEK-293 cell lysate, JAR cell lysate, HUVEC cell lysate, PC-12 cell lysate, SH-SY5Y cell lysate, C2C12 cell lysate. |
| Subcellular location: | Cytoplasm, Endomembrane system, Cell projection, lamellipodium, Cytoplasm, perinuclear region, Cell membrane, cytoskeleton. |
| Recommended Dilutions:
WB |
1:2,000-1:5,000 |
| Uniprot #: | SwissProt: P60763 Human | P60764 Mouse |
| Alternative names: | OTTMUSP00000004488 p21 Rac3 p21-Rac3 Rac1B RAC3 RAC3_HUMAN RAS related C3 botulinum substrate 3 Ras related C3 botulinum toxin substrate 3 (rho family small GTP binding protein Rac3) Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3) Ras-related C3 botulinum toxin substrate 3 Rho family small GTP binding protein Rac3 RP23-84C12.18 |
Images
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Fig1:
Western blot analysis of RAC3 on different lysates with Rabbit anti-RAC3 antibody (HA721890) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: JAR cell lysate (20 µg/Lane) Lane 3: HUVEC cell lysate (20 µg/Lane) Lane 4: PC-12 cell lysate (20 µg/Lane) Lane 5: SH-SY5Y cell lysate (15 µg/Lane) Lane 6: C2C12 cell lysate (15 µg/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721890) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of RAC3 on different lysates with Rabbit anti-RAC3 antibody (HA721890) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-RAC3 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721890) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.