ICAM2 Recombinant Rabbit Monoclonal Antibody [JE63-28]
cat.: HA721893
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE63-28 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse ICAM2 aa 1-250 / 277. |
| Positive control: | Mouse spleen tissue lysate, mouse heart tissue lysate, mouse kidney tissue lysate, rat spleen tissue lysate, mouse spleen tissue, rat spleen tissue, rat lung tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:500 |
| Uniprot #: | SwissProt: P35330 Mouse |
| Alternative names: | CD102 CD102 antigen ICAM 2 ICAM-2 Icam2 ICAM2_HUMAN Intercellular adhesion molecule 2 Ly60 |
Images
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Fig1:
Western blot analysis of ICAM2 on different lysates with Rabbit anti-ICAM2 antibody (HA721893) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse heart tissue lysate (no heat) Lane 3: Mouse kidney tissue lysate (no heat) Lane 4: Mouse kidney tissue lysate Lane 5: Mouse spleen tissue lysate (no heat) Lane 6: Rat spleen tissue lysate (no heat) Lysates/proteins at 40 µg/Lane. Predicted band size: 31 kDa Observed band size: 45-50 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721893) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-ICAM2 antibody (HA721893) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721893) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-ICAM2 antibody (HA721893) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721893) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-ICAM2 antibody (HA721893) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721893) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.