ICAM2 Recombinant Rabbit Monoclonal Antibody [JE63-28]
cat.: HA721893
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE63-28
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 31 kDa
Isotype: IgG
Immunogen: Recombinant protein within Mouse ICAM2 aa 1-250 / 277.
Positive control: Mouse spleen tissue lysate, mouse heart tissue lysate, mouse kidney tissue lysate, rat spleen tissue lysate, mouse spleen tissue, rat spleen tissue, rat lung tissue.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:500
Uniprot #: SwissProt: P35330 Mouse
Alternative names: CD102 CD102 antigen ICAM 2 ICAM-2 Icam2 ICAM2_HUMAN Intercellular adhesion molecule 2 Ly60
Images
HA721893_1.jpg Fig1: Western blot analysis of ICAM2 on different lysates with Rabbit anti-ICAM2 antibody (HA721893) at 1/1,000 dilution.

Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse heart tissue lysate (no heat)
Lane 3: Mouse kidney tissue lysate (no heat)
Lane 4: Mouse kidney tissue lysate
Lane 5: Mouse spleen tissue lysate (no heat)
Lane 6: Rat spleen tissue lysate (no heat)

Lysates/proteins at 40 µg/Lane.

Predicted band size: 31 kDa
Observed band size: 45-50 kDa

Exposure time: 1 minute 2 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721893) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721893_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-ICAM2 antibody (HA721893) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721893) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721893_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-ICAM2 antibody (HA721893) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721893) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721893_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-ICAM2 antibody (HA721893) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721893) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.