AP2S1 Recombinant Rabbit Monoclonal Antibody [JE47-37]
cat.: HA721894
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE47-37
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 17 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human AP2S1 aa 1-50 / 142.
Positive control: K-562 cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse spleen tissue lysate, rat kidney tissue lysate.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P53680 Human | P62743 Mouse | P62744 Rat
Alternative names: Adapter-related protein complex 2 sigma subunit Adaptor protein complex AP 2 subunit sigma Adaptor protein complex AP-2 subunit sigma Adaptor protein complex AP2 subunit sigma Adaptor related protein complex 2 sigma 1 subunit AI043088 AP 17 AP 2 complex subunit sigma 1 AP-2 complex subunit sigma AP17 AP17 delta AP2 complex subunit sigma 1 Ap2s1 AP2S1_HUMAN CLAPS 2 CLAPS2 Clathrin adaptor complex AP2, sigma subunit Clathrin adaptor protein AP17 Clathrin assembly protein 2 small chain Clathrin associated/assembly/adaptor protein small 2 Clathrin associated/assembly/adaptor protein small 2, 17-KD Clathrin associated/assembly/adaptor protein, small 2 (17kD) Clathrin coat assembly protein AP17 Clathrin coat associated protein AP17 Clathrin coat-associated protein AP17 HA2 17 kDa subunit MGC62945 Plasma membrane adaptor AP 2 17 kDa protein Plasma membrane adaptor AP-2 17 kDa protein Plasma membrane adaptor AP2 17 kDa protein Sigma a......
Images
HA721894_1.jpg Fig1: Western blot analysis of AP2S1 on different lysates with Rabbit anti-AP2S1 antibody (HA721894) at 1/1,000 dilution.

Lane 1: K-562 cell lysate (30 µg/Lane)
Lane 2: MCF7 cell lysate (30 µg/Lane)
Lane 3: NIH/3T3 cell lysate (30 µg/Lane)
Lane 4: PC-12 cell lysate (30 µg/Lane)
Lane 5: Mouse spleen tissue lysate (40 µg/Lane)
Lane 6: Rat kidney tissue lysate (40 µg/Lane)

Predicted band size: 17 kDa
Observed band size: 14 kDa

Exposure time: 1 minute 6 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721894) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.