LI Cadherin Recombinant Rabbit Monoclonal Antibody [JE60-34]
cat.: HA721895
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P, IF-Tissue, IF-Cell, FC
Clonality: Monoclonal
Clone number: JE60-34
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 92 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human LI Cadherin aa 1-100 / 832.
Positive control: Caco-2 cell lysate, LoVo cell lysate, Caco-2, human appendix tissue, human stomach tissue, mouse small intestine tissue.
Subcellular location: Cell membrane
Recommended Dilutions:
  WB
  IHC-P
  IF-Tissue
  IF-Cell
  FC
1:1,000
1:200-1:1,000
1:100
1:100
1:1,000
Uniprot #: SwissProt: Q12864 Human
Alternative names: BILL-cadherin CAD17_HUMAN Cadherin 16 Cadherin 16, formerly Cadherin 17 cadherin 17, LI cadherin (liver-intestine) cadherin, liver-intestine Cadherin-17 CDH16 CDH16, formerly Cdh17 FLJ26931 Formerly Cadherin 16 Formerly CDH16 HPT 1 HPT-1 cadherin human intestinal peptide-associated transporter HPT-1 human peptide transporter 1 Intestinal peptide-associated transporter HPT 1 Intestinal peptide-associated transporter HPT-1 LI-cadherin Liver Cadherin liver intestine cadherin Liver-intestine cadherin
Images
HA721895_1.jpg Fig1: Western blot analysis of LI Cadherin on different lysates with Rabbit anti-LI Cadherin antibody (HA721895) at 1/1,000 dilution.

Lane 1: Caco-2 cell lysate (20 µg/Lane)
Lane 2: LoVo cell lysate (30 µg/Lane)
Lane 3: PANC-1 cell lysate (negative) (30 µg/Lane)

Predicted band size: 92 kDa
Observed band size: 120 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721895) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721895_2.jpg Fig2: Immunocytochemistry analysis of Caco-2 cells labeling LI Cadherin with Rabbit anti-LI Cadherin antibody (HA721895) at 1/100 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LI Cadherin antibody (HA721895) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
HA721895_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-LI Cadherin antibody (HA721895) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721895) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721895_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-LI Cadherin antibody (HA721895) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721895) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721895_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-LI Cadherin antibody (HA721895) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721895) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721895_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue (negative) with Rabbit anti-LI Cadherin antibody (HA721895) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721895) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721895_7.jpg Fig7: Immunofluorescence analysis of paraffin-embedded human appendix tissue labeling LI Cadherin with Rabbit anti-LI Cadherin antibody (HA721895) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721895, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
HA721895_8.jpg Fig8: Flow cytometric analysis of Caco-2 cells labeling LI Cadherin.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA721895, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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