TFE3 Recombinant Rabbit Monoclonal Antibody [JE60-60]
cat.: HA721896
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JE60-60
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 61 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human TFE3 aa 150-499 / 575.
Positive control: U-2 OS cell lysate, 293T cell lysate, C2C12 cell lysate, mouse testis tissue lysate, A-172 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, human testis tissue, human thyroid carcinoma tissue, rat testis tissue, U-2 OS.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:1,000
1:800
1:1,000
Uniprot #: SwissProt: P19532 Human | Q64092 Mouse
Entrez Gene: 317376 Rat
Alternative names: bHLH e33 bHLHe33 Class E basic helix-loop-helix protein 33 RCCP 2 RCCP2 RCCX1 Renal cell carcinoma, papillary Tcfe 3 Tcfe3 TFE 3 Tfe3 TFE3_HUMAN TFEA Transcription factor binding to IGHM enhancer 3 Transcription factor E family, member A Transcription factor E3 Transcription factor for IgH enhancer Transcription factor for immunoglobulin heavy chain enhancer 3
Images
HA721896_1.jpg Fig1: Western blot analysis of TFE3 on different lysates with Rabbit anti-TFE3 antibody (HA721896) at 1/1,000 dilution.

Lane 1: U-2 OS cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: C2C12 cell lysate (20 µg/Lane)
Lane 4: Mouse testis tissue lysate (40 µg/Lane)
Lane 5: A-172 cell lysate (30 µg/Lane)
Lane 6: NIH/3T3 cell lysate (30 µg/Lane)
Lane 7: C6 cell lysate (30 µg/Lane)
Lane 8: PC-12 cell lysate (30 µg/Lane)

Predicted band size: 61 kDa
Observed band size: 70/80 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721896) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721896_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TFE3 antibody (HA721896) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721896) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721896_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-TFE3 antibody (HA721896) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721896) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721896_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-TFE3 antibody (HA721896) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721896) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721896_5.jpg Fig5: Flow cytometric analysis of U-2 OS cells labeling TFE3.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721896, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.