Tartrate-resistant acid phosphatase type 5 Recombinant Rabbit Monoclonal Antibody [JE35-95]
cat.: HA721897
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE35-95 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Tartrate-resistant acid phosphatase type 5 aa 126-325 / 325. |
| Positive control: | A431 cell lysate, SK-MEL-28 cell lysate, human lung tissue. |
| Subcellular location: | Lysosome |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:500 |
| Uniprot #: | SwissProt: P13686 Human |
| Alternative names: | Type 5 acid phosphatase Acid phosphatase 5 tartrate resistant Acid phosphatase 5, tartrate resistant ACP5 EC 3.1.3.2 MGC117378 PPA5_HUMAN serum band 5 tartrate-resistant acid phosphatase SPENCDI T5ap Tartrate resistant acid ATPase Tartrate resistant acid phosphatase type 5 Tartrate resistant acid phosphatase type 5 precursor Tartrate-resistant acid ATPase Tartrate-resistant acid phosphatase type 5 TR AP TR-AP TRACP 5 TRAP TrATPase Type 5 acid phosphatase |
Images
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Fig1:
Western blot analysis of Tartrate-resistant acid phosphatase type 5 on different lysates with Rabbit anti-Tartrate-resistant acid phosphatase type 5 antibody (HA721897) at 1/2,000 dilution. Lane 1: A431 cell lysate Lane 2: SK-MEL-28 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721897) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Tartrate-resistant acid phosphatase type 5 antibody (HA721897) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721897) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.