JNK1 Recombinant Rabbit Monoclonal Antibody [JE63-04]
cat.: HA721899
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell
Clonality: Monoclonal
Clone number: JE63-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 44 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human JNK1 aa 301-350 / 427.
Positive control: HeLa cell lysate, HEK-293 cell lysate, Jurkat cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, HEK-293, human colon cancer tissue, mouse brain tissue, rat brain tissue.
Subcellular location: Cytoplasm, Nucleus, Synapse.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell

1:2,000
1:500-1:2,000
1:100
Uniprot #: SwissProt: P45983 Human | Q91Y86 Mouse | P49185 Rat
Alternative names: AI849689 c Jun N terminal kinase 1 C-JUN kinase 1 c-Jun N-terminal kinase 1 EC 2.7.11.24 JNK 1 JNK JNK-46 JNK1A2 JNK21B1/2 MAP kinase 8 MAPK 8 mapk8 Mitogen activated protein kinase 8 Mitogen-activated protein kinase 8 MK08_HUMAN p54 gamma Prkm8 Protein kinase JNK1 Protein kinase, mitogen-activated, 8 SAPK 1 SAPK gamma SAPK1 Stress-activated protein kinase 1 Stress-activated protein kinase JNK1
Images
HA721899_1.jpg Fig1: Western blot analysis of JNK1 on different lysates with Rabbit anti-JNK1 antibody (HA721899) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: MCF7 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: C6 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 44 kDa
Observed band size: 44/48 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721899) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721899_2.jpg Fig2: Immunocytochemistry analysis of HEK-293 cells labeling JNK1 with Rabbit anti-JNK1 antibody (HA721899) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-JNK1 antibody (HA721899) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
HA721899_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-JNK1 antibody (HA721899) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721899) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721899_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-JNK1 antibody (HA721899) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721899) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721899_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-JNK1 antibody (HA721899) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721899) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.