Lhx2 Recombinant Rabbit Monoclonal Antibody [JE54-99]
cat.: HA721900
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE54-99 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Lhx2 aa 1-100 / 406. |
| Positive control: | A431 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P50458 Human | Q9Z0S2 Mouse | P36198 Rat |
| Alternative names: | hLhx2 Homeobox protein LH-2 Homeobox protein LH2 LH2 LHX2 LHX2_HUMAN LIM homeobox 2 LIM homeobox protein 2 LIM HOX gene 2 LIM/homeobox protein Lhx2 |
Images
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Fig1:
Western blot analysis of Lhx2 on different lysates with Rabbit anti-Lhx2 antibody (HA721900) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: Neuro-2a cell lysate (20 µg/Lane) Lane 4: Human brain tissue lysate (40 µg/Lane) Lane 5: Mouse brain tissue lysate (40 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 44 kDa Observed band size: 40-44 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721900) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.