TBX21 Recombinant Rabbit Monoclonal Antibody [JE60-20]
cat.: HA721901
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Monoclonal
Clone number: JE60-20
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 58 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human TBX21 aa 21-70 / 535.
Positive control: NK-92 cell lysate, human hodgkin's lymphoma tissue, human thymus tissue, human tonsil tissue, NK-92.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC

1:1,000
1:200
1:100
1:1,000
Uniprot #: SwissProt: Q9UL17 Human
Alternative names: T bet T box 21 T box expressed in T cells T box protein 21 T box transcription factor TBX21 T cell specific T box transcription factor T cell specific T box transcription factor T bet T PET T-box protein 21 T-box transcription factor TBX21 T-cell-specific T-box transcription factor T-bet TBET TBLYM TBX 21 Tbx21 TBX21_HUMAN TPET Transcription factor TBLYM
Images
HA721901_1.jpg Fig1: Western blot analysis of TBX21 on different lysates with Rabbit anti-TBX21 antibody (HA721901) at 1/1,000 dilution.

Lane 1: NK-92 cell lysate
Lane 2: K-562 cell lysate (negative)
Lane 3: Jurkat cell lysate (negative)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 58 kDa
Observed band size: 65 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721901) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721901_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue with Rabbit anti-TBX21 antibody (HA721901) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721901) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721901_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-TBX21 antibody (HA721901) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721901) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721901_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-TBX21 antibody (HA721901) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721901) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721901_5.jpg Fig5: Immunocytochemistry analysis of NK-92 (positive) and K-562 (negative) labeling TBX21 with Rabbit anti-TBX21 antibody (HA721901) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TBX21 antibody (HA721901) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
HA721901_6.jpg Fig6: Flow cytometric analysis of NK-92 cells labeling TBX21.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721901, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.