TBX21 Recombinant Rabbit Monoclonal Antibody [JE60-20]
cat.: HA721901
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE60-20 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TBX21 aa 21-70 / 535. |
| Positive control: | NK-92 cell lysate, human hodgkin's lymphoma tissue, human thymus tissue, human tonsil tissue, NK-92. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:1,000 1:200 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q9UL17 Human |
| Alternative names: | T bet T box 21 T box expressed in T cells T box protein 21 T box transcription factor TBX21 T cell specific T box transcription factor T cell specific T box transcription factor T bet T PET T-box protein 21 T-box transcription factor TBX21 T-cell-specific T-box transcription factor T-bet TBET TBLYM TBX 21 Tbx21 TBX21_HUMAN TPET Transcription factor TBLYM |
Images
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Fig1:
Western blot analysis of TBX21 on different lysates with Rabbit anti-TBX21 antibody (HA721901) at 1/1,000 dilution. Lane 1: NK-92 cell lysate Lane 2: K-562 cell lysate (negative) Lane 3: Jurkat cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 65 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721901) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue with Rabbit anti-TBX21 antibody (HA721901) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721901) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-TBX21 antibody (HA721901) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721901) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-TBX21 antibody (HA721901) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721901) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunocytochemistry analysis of NK-92 (positive) and K-562 (negative) labeling TBX21 with Rabbit anti-TBX21 antibody (HA721901) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TBX21 antibody (HA721901) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig6:
Flow cytometric analysis of NK-92 cells labeling TBX21. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721901, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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