ABHD5 Recombinant Rabbit Monoclonal Antibody [JE60-62]
cat.: HA721903
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE60-62 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ABHD5 aa 151-200 / 349. |
| Positive control: | NIH/3T3 cell lysate, mouse pancreas tissue lysate, mouse kidney tissue lysate, NIH/3T3. |
| Subcellular location: | Cytoplasm, Lipid droplet. |
| Recommended Dilutions:
WB FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q8WTS1 Human | Q9DBL9 Mouse |
| Alternative names: | 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 ABHD5 ABHD5_HUMAN Abhydrolase domain containing 5 Abhydrolase domain containing protein 5 Abhydrolase domain-containing protein 5 CDS CGI 58 CGI58 CGI58 protein IECN2 Lipid droplet-binding protein CGI-58 MGC8731 NCIE2 |
Images
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Fig1:
Western blot analysis of ABHD5 on different lysates with Rabbit anti-ABHD5 antibody (HA721903) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: Mouse pancreas tissue lysate (40 µg/Lane) Lane 3: Mouse kidney tissue lysate (40 µg/Lane) Predicted band size: 39 kDa Observed band size: 43 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721903) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of NIH/3T3 cells labeling ABHD5. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721903, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.