| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE33-21 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 10 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CCL4 / MIP-1 beta aa 1-50 / 92. |
| Positive control: | THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate, RAW264.7 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P13236 Human | Q8NHW4 Human | P14097 Mouse |
| Alternative names: | MIP 1 beta Secreted protein G 26 ACT 2 ACT-2 ACT2 AT744.1 AT744.2 C C motif chemokine 4 C C motif chemokine 4 like C C motif chemokine ligand 4 like 1 C C motif chemokine ligand 4 like 2 CC chemokine ligand 4 CC chemokine ligand 4L1 CC chemokine ligand 4L1d2 CC chemokine ligand 4L2 CCL4 CCL4_HUMAN ccl4l 1 CCL4L CCL4L1 Chemokine (C C motif) ligand 4 Chemokine (C C motif) ligand 4 like 1 Chemokine (C C motif) ligand 4 like 1, telomeric Chemokine (C C motif) ligand 4 like 2 Chemokine CC Motif Ligand 4 G 26 G 26 T lymphocyte secreted protein G-26 T-lymphocyte-secreted protein HC21 Immune activation 2 LAG 1 LAG-1 LAG1 Lymphocyte activation gene 1 Lymphocyte activation gene 1 protein Macrophage inflammatory protein 1 beta Macrophage inflammatory protein 1-beta Macrophage inflammatory protein 1b2 MGC104418 MGC126025 MGC126026 MIP-1-beta MIP-1-beta(1-69) MIP-1-beta(3-69) MIP1 beta MIP1B MIP1B1 Monocyt...... |
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Fig1:
Western blot analysis of CCL4 / MIP-1 beta on different lysates with Rabbit anti-CCL4 / MIP-1 beta antibody (HA721904) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 1 minute 10 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721904) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |