CCL4/MIP-1 beta Recombinant Rabbit Monoclonal Antibody [JE33-21]
cat.: HA721904
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB
Clonality: Monoclonal
Clone number: JE33-21
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 12 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CCL4/MIP-1 beta aa 1-50 / 92.
Positive control: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate, RAW264.7 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate.
Subcellular location: Secreted.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P13236 Human | Q8NHW4 Human | P14097 Mouse
Alternative names: MIP 1 beta Secreted protein G 26 ACT 2 ACT-2 ACT2 AT744.1 AT744.2 C C motif chemokine 4 C C motif chemokine 4 like C C motif chemokine ligand 4 like 1 C C motif chemokine ligand 4 like 2 CC chemokine ligand 4 CC chemokine ligand 4L1 CC chemokine ligand 4L1d2 CC chemokine ligand 4L2 CCL4 CCL4_HUMAN ccl4l 1 CCL4L CCL4L1 Chemokine (C C motif) ligand 4 Chemokine (C C motif) ligand 4 like 1 Chemokine (C C motif) ligand 4 like 1, telomeric Chemokine (C C motif) ligand 4 like 2 Chemokine CC Motif Ligand 4 G 26 G 26 T lymphocyte secreted protein G-26 T-lymphocyte-secreted protein HC21 Immune activation 2 LAG 1 LAG-1 LAG1 Lymphocyte activation gene 1 Lymphocyte activation gene 1 protein Macrophage inflammatory protein 1 beta Macrophage inflammatory protein 1-beta Macrophage inflammatory protein 1b2 MGC104418 MGC126025 MGC126026 MIP-1-beta MIP-1-beta(1-69) MIP-1-beta(3-69) MIP1 beta MIP1B MIP1B1 Monocyt......
Images
HA721904_1.jpg Fig1: Western blot analysis of CCL4/MIP-1 beta on different lysates with Rabbit anti-CCL4/MIP-1 beta antibody (HA721904) at 1/1,000 dilution.

Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: RAW264.7 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 10 kDa
Observed band size: 10 kDa

Exposure time: 1 minute 10 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721904) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.