ATAD2 Recombinant Rabbit Monoclonal Antibody [JE34-52]
cat.: HA721905
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE34-52 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 158 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ATAD2 aa 31-130 / 1,390. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, T-47D cell lysate, HT-29 cell lysate, Saos- 2 cell lysate, HepG2 cell lysate, Jurkat cell lysate, human testis tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:2,000 |
| Uniprot #: | SwissProt: Q6PL18 Human |
| Alternative names: | AAA nuclear coregulator cancer-associated protein AAA nuclear coregulator, cancer-associated ANCCA Atad2 ATAD2_HUMAN ATPase family AAA domain containing 2 ATPase family AAA domain containing protein 2 ATPase family AAA domain-containing protein 2 CT137 DKFZp667N1320 L16 MGC131938 MGC142216 MGC29843 MGC5254 PRO2000 |
Images
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Fig1:
Western blot analysis of ATAD2 on different lysates with Rabbit anti-ATAD2 antibody (HA721905) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: T-47D cell lysate Lane 4: HT-29 cell lysate Lane 5: Saos- 2 cell lysate Lane 6: HepG2 cell lysate Lane 7: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 158 kDa Observed band size: 180 kDa Exposure time: 4 minutes 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721905) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ATAD2 on different lysates with Rabbit anti-ATAD2 antibody (HA721905) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD ATAD2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 158 kDa Observed band size: 180 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721905) at 1/1,000 dilution was used in primary antibody diluent at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ATAD2 antibody (HA721905) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721905) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.