RNF8 Recombinant Rabbit Monoclonal Antibody [JE56-43]
cat.: HA721907
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE56-43 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human RNF8 aa 416-465 / 485. |
| Positive control: | Human brain tissue lysates, human brain tissue. |
| Subcellular location: | Chromosome, Cytoplasm, Nucleus, Telomere. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:500 |
| Uniprot #: | SwissProt: O76064 Human |
| Alternative names: | C3HC4 type zinc finger protein E3 ubiquitin protein ligase RNF8 E3 ubiquitin-protein ligase RNF8 FLJ12013 KIAA0646 Ring finger protein (C3HC4 type) 8 RING finger protein 8 Ring finger protein 8, E3 ubiquitin protein ligase RNF 8 RNF8 RNF8_HUMAN UBC13/UEV interacting ring finger protein |
Images
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Fig1:
Western blot analysis of RNF8 on human brain tissue lysates with Rabbit anti-RNF8 antibody (HA721907) at 1/2,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 56 kDa Observed band size: 63 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721907) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-RNF8 antibody (HA721907) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721907) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.