HA721908

SA2 Recombinant Rabbit Monoclonal Antibody [JE57-34]

cat.: HA721908

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Monkey
Applications:	WB, IHC-P, IF-Cell
Clonality:	Monoclonal
Clone number:	JE57-34

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 141 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human SA2 aa 982-1,231 / 1,231.
Positive control:	HeLa cell lysate, A431 cell lysate, PANC-1 cell lysate, HepG2 cell lysate, Jurkat cell lysate, MCF7 cell lysate, K-562 cell lysate, HCT 116 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, human breast cancer tissue, human spleen tissue, human tonsil tissue, mouse spleen tissue, rat spleen tissue, RAW264.7.
Subcellular location:	Nucleus. Chromosome.
Recommended Dilutions: WB IHC-P IF-Cell	1:2,000 1:500-1:2,000 1:100
Uniprot #:	SwissProt: Q8N3U4 Human \| O35638 Mouse Entrez Gene: 313304 Rat
Alternative names:	bA517O1.1 Cohesin Subunit SA 2 Cohesin subunit SA-2 DKFZp686P168 DKFZp781H1753 FLJ25871 SA 2 SA-2 SA2 SCC3 homolog 2 SCC3B STAG 2 stag2 STAG2_HUMAN Stromal antigen 2

Images

Fig1: Western blot analysis of SA2 on different lysates with Rabbit anti-SA2 antibody (HA721908) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: PANC-1 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF7 cell lysate
Lane 7: K-562 cell lysate
Lane 8: HCT 116 cell lysate
Lane 9: COS-1 cell lysate
Lane 10: NIH/3T3 cell lysate
Lane 11: RAW264.7 cell lysate
Lane 12: C6 cell lysate
Lane 13: mouse spleen tissue lysate
Lane 14: rat spleen tissue lysate

Cell lysates/proteins at 20 µg/Lane.
Tissues lysates/proteins at 40 µg/Lane.

Predicted band size:141 kDa
Observed band size: 141 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721908) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-SA2 antibody (HA721908) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721908) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-SA2 antibody (HA721908) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721908) at 1/2.000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-SA2 antibody (HA721908) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721908) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-SA2 antibody (HA721908) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721908) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-SA2 antibody (HA721908) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721908) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Immunocytochemistry analysis of RAW264.7 cells labeling SA2 with Rabbit anti-SA2 antibody (HA721908) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SA2 antibody (HA721908) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.