Lrp2 / Megalin Recombinant Rabbit Monoclonal Antibody [PSH02-91]
cat.: HA721910
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, IF-Tissue, WB |
| Clonality: | Monoclonal |
| Clone number: | PSH02-91 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 522 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Lrp2 aa 2,500-3,200 / 4,655. |
| Positive control: | Human kidney tissue lysates, human kidney tissue, human liver tissue. |
| Subcellular location: | Apical cell membrane, Endosome lumen, Membrane, coated pit, Cell projection, dendrite, axon. |
| Recommended Dilutions:
IHC-P IF-Tissue WB |
1:1,000 1:50 1:2,000 |
| Uniprot #: | SwissProt: P98164 Human |
| Alternative names: | Calcium sensor protein DBS Glycoprotein 330 gp330 Heymann nephritis antigen homolog Low-density lipoprotein receptor-related protein 2 LRP-2 Lrp2 LRP2_HUMAN Megalin |
Images
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Fig1:
Western blot analysis of Lrp2 / Megalin on human kidney tissue lysates with Rabbit anti-Lrp2 / Megalin antibody (HA721910) at 1/2,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721910) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Lrp2 / Megalin antibody (HA721910) at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721910) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue (negative) with Rabbit anti-Lrp2 / Megalin antibody (HA721910) at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721910) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Lrp2 / Megalin with Rabbit anti-Lrp2 / Megalin antibody (HA721910) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721910, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.