PPP2R1B Recombinant Rabbit Monoclonal Antibody [PSH02-92]
cat.: HA721911
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH02-92 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PPP2R1B aa 1-601 / 601. |
| Positive control: | Caco-2 cell lysate, HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human liver tissue, mouse liver tissue, mouse testis tissue, rat liver tissue, rat testis tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P30154 Human | Q7TNP2 Mouse | Q4QQT4 Rat |
| Alternative names: | 2AAB_HUMAN beta isoform of regulatory subunit A, protein phosphatase 2 PP2A A beta PP2A subunit A isoform PR65-beta PP2A subunit A isoform R1-beta PP2A, subunit A, PR65 beta isoform PP2A, subunit A, R1 beta isoform PPP2R1B PPP4R1 PR65B protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform Protein phosphatase 2, regulatory subunit A, beta Protein phosphatase 2, structural/regulatory subunit A, beta protein phosphatase 2, structural/regulatory subunit A, beta isoform serine/threonine protein phosphatase 2A 65 kDa regulatory subunit A, beta isoform serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, beta isoform Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform |
Images
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Fig1:
Western blot analysis of PPP2R1B on different lysates with Rabbit anti-PPP2R1B antibody (HA721911) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: Caco-2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lane 5: RAW264.7 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721911) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PPP2R1B antibody (HA721911) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721911) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-PPP2R1B antibody (HA721911) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721911) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-PPP2R1B antibody (HA721911) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721911) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-PPP2R1B antibody (HA721911) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721911) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-PPP2R1B antibody (HA721911) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721911) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Western blot analysis of PPP2R1B on different lysates with Rabbit anti-PPP2R1B antibody (HA721911) at 1/2,000 dilution. Lane 1: HeLa-si NT cell lysate (10 µg/Lane) Lane 2: HeLa-si PPP2R1B cell lysate (10 µg/Lane) Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721911) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.