Lamin B Receptor / LBR Recombinant Rabbit Monoclonal Antibody [PSH02-93]
cat.: HA721912
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH02-93 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Lamin B Receptor/LBR aa 1-500 / 615. |
| Positive control: | HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, HEK-293, human small intestine tissue, human testis tissue. |
| Subcellular location: | Nucleus inner membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:2,000 1:1,000-10,000 1:2,000 1:1,000 |
| Uniprot #: | SwissProt: Q14739 Human |
| Alternative names: | DHCR 14B DHCR14B Integral nuclear envelope inner membrane protein Lamin-B receptor LBR LBR_HUMAN LMN 2R LMN2R MGC9041 PHA PRO0650 |
Images
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Fig1:
Western blot analysis of Lamin B Receptor / LBR on different lysates with Rabbit anti-Lamin B Receptor / LBR antibody (HA721912) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 71 kDa Observed band size: 58 kDa Exposure time: Lane 1-2: 1 minute 2 seconds; Lane 3: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721912) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HEK-293 cells labeling Lamin B Receptor / LBR with Rabbit anti-Lamin B Receptor / LBR antibody (HA721912) at 1/2,000 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Lamin B Receptor / LBR antibody (HA721912) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Lamin B Receptor / LBR antibody (HA721912) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721912) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Lamin B Receptor / LBR antibody (HA721912) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721912) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of HEK-293 cells labeling Lamin B Receptor / LBR. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721912, 1:1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.