Axin1 Recombinant Rabbit Monoclonal Antibody [PSH03-00]
cat.: HA721919
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH03-00 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 95.6 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Axin1 aa 1-400 / 862. |
| Positive control: | SW480 cell lysate, HeLa cell lysate, 293T cell lysate, K-562 cell lysate, Huh7 cell lysate, human colon cancer tissue. |
| Subcellular location: | Cytoplasm. Nucleus. Membrane. Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:500-1:2,000 |
| Uniprot #: | SwissProt: O15169 Human |
| Alternative names: | AI316800 AXIN Axin 1 Axin-1 axin1 AXIN1_HUMAN Axis inhibition protein 1 Axis Inhibitor 1 Fu fused Fused, mouse, homolog of hAxin Kb Ki kinky knobbly MGC132911 MGC52315 PPP1R49 Protein Fused Protein phosphatase 1 regulatory subunit 49 |
Images
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Fig1:
Western blot analysis of Axin1 on different lysates with Rabbit anti-Axin1 antibody (HA721919) at 1/2,000 dilution. Lane 1: SW480 cell lysate (10 µg/Lane) Lane 2: HeLa cell lysate (10 µg/Lane) Lane 3: 293T cell lysate (10 µg/Lane) Lane 4: K-562 cell lysate (10 µg/Lane) Lane 5: Huh7 cell lysate (10 µg/Lane) Predicted band size: 96 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721919) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Axin1 on different lysates with Rabbit anti-Axin1 antibody (HA721919) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Axin1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 96 kDa Observed band size: 110 kDa Exposure time: 140 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721919) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Axin1 antibody (HA721919) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721919) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.